January 2021

What is C banding?- Requirement, Process, Protocol and Applications

Chromosome banding / By

“The C banding or C bands is a technique utilized in karyotyping which stains especially the centromeric region, stronger than other regions.”   Do you know? The centromere region of chromosomes is a repetitive DNA region, condensed and the non-active region.  It distinguished two arms from each other.  Centromere helps in the separation of chromosomes or …

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What is Q banding?- Requirement, Protocol and Applications

What is Q banding?- Requirement, Protocol and Applications

Chromosome banding / By

“The Q banding or quinacrine banding technique in which the fluorescence dye quinacrine binds or intercalates with the DNA and gives different banding patterns on chromosomes.”  The technique is also known as QFQ banding- Q-bands using fluorescence quinacrine and was first explained by Caspersson and co-workers.   Related article: 20 Common karyotyping (or Chromosomal) Abnormalities. What …

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What is T banding?- Requirement, Protocol and Applications

What is T banding?- Requirement, Protocol and Applications

Chromosome banding / By

“The t banding or t bands is a technique utilized in karyotyping which stains especially the telomeric region, stronger than other regions.”   Karyotyping is a technique employed to study chromosomes include steps such as sample collection, cell culture, cell harvesting, microscopy and results analysis.   Diploid human cells have 23 pairs of chromosomes, revealed through karyotyping.  …

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what is the role of macro photography in karyotyping?

What is the role of Macro photography in Cytogenetics?

Karyotyping / By

Photography, especially the macro photography lens is commonly used to investigate smaller living organisms and objects like chromosomes or chromosome bands. Do you know? Wide-angle lens, portrait lens and macro lenses are three common lenses used for various types of photography.  Cytogenetic technique such as karyotyping and FISH requires state of the art microscopy set …

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Why Karyotyping is performed using Monocytes only?

Why Karyotyping is performed using Monocytes only?

Karyotyping / By

Monocytes are a special type of bone marrow-derived leukocytes commonly cultured in karyotyping procedure.  Karyotyping is a type of cytogenetic technique used commonly for observing chromosomes. Its objective is to investigate chromosomes for finding abnormalities associated with different genetic conditions.  It’s a traditional yet popular and versatile technique, scientists use for encountering various defects and …

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