“The cell culture media is a composition of nutrients like amino acids, carbohydrates, vitamins and other ingredients essential for the growth of cells in vitro.” Culture allows scientists to learn more about cells and related things! The cell culture is an aseptic condition to isolate cells followed by growing them artificially, in vitro processes using …
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Photography, especially the macro photography lens is commonly used to investigate smaller living organisms and objects like chromosomes or chromosome bands. Do you know? Wide-angle lens, portrait lens and macro lenses are three common lenses used for various types of photography. Cytogenetic technique such as karyotyping and FISH requires state of the art microscopy set …
What is the role of Macro photography in Cytogenetics? Read More »
Monocytes are a special type of bone marrow-derived leukocytes commonly cultured in karyotyping procedure. Karyotyping is a type of cytogenetic technique used commonly for observing chromosomes. Its objective is to investigate chromosomes for finding abnormalities associated with different genetic conditions. It’s a traditional yet popular and versatile technique, scientists use for encountering various defects and …
Why Karyotyping is performed using Monocytes only? Read More »
Sodium heparin is the best anticoagulant agent that prevents coagulation of blood henceforth sodium heparin-containing tubes are commonly used for the sample collection for karyotyping. A very important procedure in cytogenetics and genetics is sample collection. The quality of results relies on the quality and conditions of the sample. Biological sample collection required special treatment, …
Why only Sodium Heparin Tubes are used to collect Samples for Karyotyping? Read More »
When a part of chromosome breaks or is deleted and reinserted at another location on a chromosome is known as a chromosome translocation. Conventional karyotyping is a technique commonly employed to study translocations. Any sudden change, alteration or undesirable alteration in a gene or chromosome might be harmful to our health. “Mutation” is a common …
What is Chromosomal Translocation?- Definition, Mechanism and Types Read More »
A Cytogenetic technique is known as karyotyping determines the number and structure of chromosomes. Based on the size and shape of chromosomes and location of the centromere it is divided into metacentric, acrocentric, telocentric types. Two important parts of chromosomes are the arms and the centromere. The location of the centromere varies from chromosome to …
Metacentric, Acrocentric and Telocentric Chromosomes Read More »
“Karyotyping is a technique based on cell culture; employed to detect chromosomal alterations but can’t detect variations at the DNA level.” Karyotyping is a very traditional, manual and primitive technique, employed in recent times too for the detection of various genetic anomalies. Genetic problems/diseases or anomalies are arisen due to mutations/alterations or variations in either …
Limitations of Karyotyping- What can’t be detected? Read More »
The process of separating and observing chromosomes is known as karyotyping (collectively) includes cell culture, incubation, cell harvesting and slide preparation. Genetic techniques are of two types; cytogenetics and molecular genetics. Karyotyping, fluorescence in situ hybridization and chromosome microarray like techniques are categorized in cytogenetic techniques while techniques like Polymerase chain reaction, DNA sequencing and …
Explaining the whole karyotyping technique and procedure Read More »