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What is Chromosomal Translocation?- Definition, Mechanism and Types

Karyotyping, Karyotyping results / By

When a part of chromosome breaks or is deleted and reinserted at another location on a chromosome is known as a chromosome translocation. Conventional karyotyping is a technique commonly employed to study translocations.  Any sudden change, alteration or undesirable alteration in a gene or chromosome might be harmful to our health. “Mutation” is a common …

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Type of Chromosomes in Normal Karyotype- Metacentric, Acrocentric and Telocentric Chromosomes

Metacentric, Acrocentric and Telocentric Chromosomes

Karyotyping / By

A Cytogenetic technique is known as karyotyping determines the number and structure of chromosomes. Based on the size and shape of chromosomes and location of the centromere it is divided into metacentric, acrocentric, telocentric types.  Two important parts of chromosomes are the arms and the centromere. The location of the centromere varies from chromosome to …

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Limitations of Karyotyping- What can’t be detected?

Karyotyping / By

“Karyotyping is a technique based on cell culture; employed to detect chromosomal alterations but can’t detect variations at the DNA level.”  Karyotyping is a very traditional, manual and primitive technique, employed in recent times too for the detection of various genetic anomalies. Genetic problems/diseases or anomalies are arisen due to mutations/alterations or variations in either …

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Explaining the whole karyotyping technique and procedure

1 Comment / Karyotyping / By

The process of separating and observing chromosomes is known as karyotyping (collectively) includes cell culture, incubation, cell harvesting and slide preparation.  Genetic techniques are of two types; cytogenetics and molecular genetics. Karyotyping, fluorescence in situ hybridization and chromosome microarray like techniques are categorized in cytogenetic techniques while techniques like Polymerase chain reaction, DNA sequencing and …

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ISCN- International System for Human Chromosome Nomenclature for Karyotyping

1 Comment / Karyotyping / By

ISCN- International System for Human Chromosome Nomenclature was developed to denote various chromosomal anomalies to make results conclusive universally.  The manual of denotation is based on the outcomes of 1977’s standing committee met in Stockholm.  Karyotyping is a manual process to perform as well as to make and interpret results. Unlike other techniques a vast …

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GTG-Banding (G-bands by Trypsin using Giemsa) for karyotyping

Karyotyping / By

GTG banding also is known as the Giemsa-trypsin-Giemsa banding technique is employed during karyotyping to investigate chromosomes and chromosomal anomalies.  Chromosomal anomalies like structural aberrations or numerical aberrations are commonly encountered using different types of banding techniques. GTG banding, R-banding, NOR-banding and Q- banding are popular techniques used routinely.  However, the GTG banding technique used …

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