What is FBS (Fetal Bovine Serum)?- Definition, Processing, Benefits, Applications and Limitations

What is FBS (Fetal Bovine Serum)?- Definition, Processing, Benefits, Applications and Limitations

A type of natural growth media-rich in nutrients, extracted from cattle meatpacking; as a byproduct is known as fetal bovine serum.  Tissue culture or cell culture is one of the most valuable, important and advantageous methods for the plant, animal and bacterial culture. Culture or cell culture is an artificial method to grow cells outside …

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Cell culture: Definition, types, media and examples

Cell Culture: Definition, Types, Media and Examples

“The cell culture media is a composition of nutrients like amino acids, carbohydrates, vitamins and other ingredients essential for the growth of cells in vitro.”  Culture allows scientists to learn more about cells and related things! The cell culture is an aseptic condition to isolate cells followed by growing them artificially, in vitro processes using …

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What is R banding?

What is R banding?- Principle, Requirements, Process, Protocol and Applications

The R banding is reverse banding or reverse Giemsa banding used to stain the regions of GC, a complement to G bands.  We are exploring varied chromosome banding techniques that are used for various clinical investigations and other applications. So far we have discussed, GTG banding, FQF banding, C banding, NOR banding and T banding.  …

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What is M banding?- Requirement, Process, Protocol and Applications

What is M banding?- Requirement, Process, Protocol and Applications

M-Bands or M Banding is a high-resolution chromosome banding technique that finds cryptic chromosomal rearrangements such as inversion, translocation, deletion or duplication at the chromosome level.  Banding techniques have a significant role in cytogenetics especially in karyotyping. The whole cytogenetic studies are conducted around chromosomes and to encounter chromosomal anomalies. Many techniques and improvements over …

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What is NOR Banding?- Requirement, Process, Protocol and Applications

What is NOR Banding?- Requirement, Process, Protocol and Applications

NOR banding or nucleolar organizing region banding technique stains acrocentric chromosomes efficiently such as 13, 14, 15, 21 and 22.  Banding techniques are specialized procedures of chemical treatment and staining using various stains in order to study chromosomes. So far on this blog, we have explained many different banding techniques like GTG banding, QFQ banding, …

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What is an Isochromosome?- Definition, Mechanism and Aberrations

What is an Isochromosome?- Definition, Mechanism and Aberrations

“Isochromosome occurs by two common events simultaneously duplication of entire one arm and loss of another one. It causes various chromosomal aberrations. On this website, we are covering interesting articles especially focusing on cytogenetic, karyotyping, chromosomes and related abnormalities. Our cytogenetic expert team collects and shares every piece of information possibly present or not.  We …

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What is C banding?- Requirement, Process, Protocol and Applications

“The C banding or C bands is a technique utilized in karyotyping which stains especially the centromeric region, stronger than other regions.”   Do you know? The centromere region of chromosomes is a repetitive DNA region, condensed and the non-active region.  It distinguished two arms from each other.  Centromere helps in the separation of chromosomes or …

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