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What is M banding?- Requirement, Process, Protocol and Applications

What is M banding?- Requirement, Process, Protocol and Applications

M-Bands or M Banding is a high-resolution chromosome banding technique that finds cryptic chromosomal rearrangements such as inversion, translocation, deletion or duplication at the chromosome level.  Banding techniques have a significant role in cytogenetics especially in karyotyping. The whole cytogenetic studies are conducted around chromosomes and to encounter chromosomal anomalies. Many techniques and improvements over […]

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What is NOR Banding?- Requirement, Process, Protocol and Applications

What is NOR Banding?- Requirement, Process, Protocol and Applications

NOR banding or nucleolar organizing region banding technique stains acrocentric chromosomes efficiently such as 13, 14, 15, 21 and 22.  Banding techniques are specialized procedures of chemical treatment and staining using various stains in order to study chromosomes. So far on this blog, we have explained many different banding techniques like GTG banding, QFQ banding,

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What is an Isochromosome?- Definition, Mechanism and Aberrations

What is an Isochromosome?- Definition, Mechanism and Aberrations

“Isochromosome occurs by two common events simultaneously duplication of entire one arm and loss of another one. It causes various chromosomal aberrations. On this website, we are covering interesting articles especially focusing on cytogenetic, karyotyping, chromosomes and related abnormalities. Our cytogenetic expert team collects and shares every piece of information possibly present or not.  We

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What is C banding?- Requirement, Process, Protocol and Applications

“The C banding or C bands is a technique utilized in karyotyping which stains especially the centromeric region, stronger than other regions.”   Do you know? The centromere region of chromosomes is a repetitive DNA region, condensed and the non-active region.  It distinguished two arms from each other.  Centromere helps in the separation of chromosomes or

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What is Q banding?- Requirement, Protocol and Applications

What is Q banding?- Requirement, Protocol and Applications

“The Q banding or quinacrine banding technique in which the fluorescence dye quinacrine binds or intercalates with the DNA and gives different banding patterns on chromosomes.”  The technique is also known as QFQ banding- Q-bands using fluorescence quinacrine and was first explained by Caspersson and co-workers.   Related article: 20 Common karyotyping (or Chromosomal) Abnormalities. What

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What is T banding?- Requirement, Protocol and Applications

What is T banding?- Requirement, Protocol and Applications

“The t banding or t bands is a technique utilized in karyotyping which stains especially the telomeric region, stronger than other regions.”   Karyotyping is a technique employed to study chromosomes include steps such as sample collection, cell culture, cell harvesting, microscopy and results analysis.   Diploid human cells have 23 pairs of chromosomes, revealed through karyotyping. 

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what is the role of macro photography in karyotyping?

What is the role of Macro photography in Cytogenetics?

Photography, especially the macro photography lens is commonly used to investigate smaller living organisms and objects like chromosomes or chromosome bands. Do you know? Wide-angle lens, portrait lens and macro lenses are three common lenses used for various types of photography.  Cytogenetic technique such as karyotyping and FISH requires state of the art microscopy set

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what is missing chromosome?

What is Missing Chromosome?

An error in meiosis causes a change in the number of chromosomes in which sometimes a chromosome got missing causing a variety of health problems. Those are also known as missing chromosome disorders, for example, Turner syndrome.  Numerical chromosomal abnormalities are significantly involved in varieties of health conditions due to loss or gain of genetic

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Are YY chromosomes possible?

Are YY Chromosomes Possible?

Two Y chromosomes are present in a chromosomal abnormality known as XYY or Jacob’s syndrome, however, only YY chromosomes in a genome aren’t reported to date. X and Y chromosomes are two genetic material repositories significantly important for humans. The Y chromosome is present in males while the X chromosome is present in males and

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